Reinhild Kappelhoff

Lab Manager, Research Associate

Sustainability Coordinator – Green Labs Program

Centre for Blood Research
University of British Columbia
2350 Health Sciences Mall
Vancouver, BC, V6T 1Z3, Canada

Office: #4.402. Lab: #4.420, Bench 9.
Phone: +1-604-822-8233 (office), +1-604-822-3561 (lab)
Fax: +1-604-822-7742
Email: reinhild.kappelhoff@ubc.ca

UBC Energy Challenge 2016:

Shut-the-Sash Video featuring TEAM SHELDON:

 TEAM SHELDON – Shut the sash

Education

  • since 07/2005 – Research Associate and Labmanager in Prof. Dr. Christopher M. Overall’s Lab
  • 02/2003 – 06/2005 – Postdoctoral Fellow at the University of British Columbia, Faculty of Dentistry, Department of Oral Biological and Medical Sciences, Prof. Dr. Christopher M. Overall’s Lab
  • 02/1999 – 01/2003 – Doctoral dissertation (Dr. rer. nat.) – “Activation mechanism of Proastacin”, Institute of Zoophysiology, Molecular Physiology, University of Muenster, Germany, Prof. Dr. Walter Stoecker
  • 07/1997 – 01/1999 – Diploma thesis (dipl. biol.) – “Bacterial expression of the zinc-endopeptidase Astacin”, Institute of Zoophysiology, Molecular Physiology, University of Muenster, Germany, Prof. Dr. Walter Stoecker
  • 09/1992 – 06/1997 – Biology studies at the University of Muenster, Germany (Major in Zoology and Microbiology)

Publications

  • Eckhard U, Huesgen PF, Schilling O, Bellac CL, Butler GS, Cox JH, Dufour A, Goebeler V, Kappelhoff R, auf dem Keller U, Klein T, Lange PL, Marino G, Morrison CJ, Prudova A, Rodriguez D, Starr AE, Wang Y, and Overall, C.M.  Active Site Specificity of the Matrix Metalloproteinase Family: Proteomic Identification of 4,300 Cleavage Sites by Nine MMPs Explored with Structural and Synthetic Peptide Cleavage Analyses. Matrix Biology, 2015 Sep 25. pii: S0945-053X(15)00129-8.
  • Fortelny N, Cox JH, Kappelhoff R, Starr AE, Lange PF, Pavlidis P, Overall CM. Network analyses reveal pervasive functional regulation between proteases in the human protease web. PLoS Biol. 2014 May 27; 12(5):e1001869.
  • Korpos E, Kadri N, Kappelhoff R, Wegner J, Overall CM, Weber E, Holmberg D, Cardell S, Sorokin L. The peri-islet basement membrane, a barrier to infiltrating leukocites in type 1 diabetes in mouse and human. Diabetes. 2013 Feb; 62(2):531-42.
  • Antunes LC, Wang M, Andersen SK, Ferreira RB, Kappelhoff R, Han J, Borchers CH, Finlay BB. Repression of Salmonella phoP expression by small molecules from physiological bile. J Bacteriol. 2012 May; 194(9):2286-96.
  • Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C. Microarray and proteomic analysis of breast cancer cell and osteoblast co-cultures: role of osteoblast matrix metalloproteinase (MMP)-13 in bone metastasis. J Biol Chem. 2011 Sep 30;286(39):34271-85.
  • Becker-Pauly C, Barré O, Schilling O, Auf dem Keller U, Ohler A, Broder C, Schütte A, Kappelhoff R, Stöcker W, Overall CM. Proteomic analyses reveal an acidic prime side specificity for the astacin metalloprotease family reflected by physiological substrates. Mol Cell Proteomics. 2011 Sep;10(9):M111.009233.
  • Cox JH, Starr AE, Kappelhoff R, Yan R, Roberts CR, Overall CM. Matrix metalloproteinase 8 deficiency in mice exacerbates inflammatory arthritis through delayed neutrophil apoptosis and reduced caspase 11 expression. Arthritis Rheum. 2010 Dec; 62(12):3645-55.
  • auf dem Keller U, Bellac CL, Li Y, Lou Y, Lange PF, Ting R, Harwig C, Kappelhoff R, Dedhar S, Adam MJ, Ruth TJ, Benard F, Perrin DM, Overall CM. Novel matrix metalloproteinase inhibitor [18F]marimastat-aryltrifluoroborate as a probe for in vivo positron emission tomography imaging in cancer. Cancer Res. 2010 Oct 1;70(19):7562-9.
  • Guevara T, Yiallouros I, Kappelhoff R, Bissdorf S, Stocker W, Gomis-Ruth FX. Proenzyme structure and activation of astacin metallopeptidase. J Biol Chem. 2010 Apr 30;285(18):13958-65.
  • Kappelhoff R, auf dem Keller U, Overall CM. Analysis of the degradome with the CLIP-CHIP microarray. Methods Mol Biol. 2010;622:175-93.
  • Kappelhoff R, Overall C. The CLIP-CHIP oligonucleotide microarray: dedicated array for analysis of all protease, nonproteolytic homolog, and inhibitor gene transcripts in human and mouse. Curr Protoc Protein Sci. 2007 Aug;Chapter 21:Unit 21.19.
  • Overall CM, Tam EM, Kappelhoff R, Connor A, Ewart T, Morrison CJ, Puente X, Lopez-Otin C, Seth A. Protease degradomics: mass spectrometry discovery of protease substrates and the CLIP-CHIP, a dedicated DNA microarray of all human proteases and inhibitors. Biol Chem. 2004, 385(6):493-504.
  • Kappelhoff R, Yiallouros I, Schilling O, Wegmann F, Helms, MW, Auge A, Brachtendorf G, Grosse Berkhoff E, Beermann B, Hinz H-J, Konig S, Peter-Katalinic J and Stocker W. Activation mechanism of pro-astacin. Role of the propeptide, tryptic and autoproteolytc cleavage and importance of precise amino-terminal processing. J. Mol. Biol. 2002, 224, 237-46.

Talks

  • Kappelhoff, R., and Overall, C.M. “It’s easy being green.” 12th Pacific Coast Protease Spring School, Desert Hot Springs, CA, USA, May 3-6, 2015.
  • Kappelhoff, R., Barré, O., and Overall, C.M. “Proteomic Identification of Natural Substrates for Matriptase-2. 9th Pacific Coast Protease Spring School, Temecula, California, USA, April 2012.
  • Kappelhoff, R., Butler, GS, and Overall, CM. “What’s Up With Wild-type? Comparing Wild-type and Knock-out Mice on Transcriptomic and Proteomic Level”. 8th Pacific Coast Protease Spring School, Borrego Springs, California, USA, April 2011.
  • Kappelhoff, R. and Overall, CM. “THE CLIP-CHIP Microarray: A tool for profiling and comparing protease and inhibitor gene expression in human and mouse tissues”. Pacific Coast Protease Spring School, Borrego Springs, California, USA, April 2010.
  • Kappelhoff, R. THE CLIP-CHIP Microarray: Tissue profiling of all Proteases, Inhibitors and non-proteolytic homologs in Human and Mouse. Molecular Life Sciences Seminar, Jacobs University Bremen, Germany, Oct 06, 2008.
  • Kappelhoff, R., Puente, X.S., Wilson, C.H., Seth, A., Lopez-Otin, C., and Overall, C.M. The CLIP-CHIP™ microarray: Tissue profiling of all proteases, non-proteolytic homologs and inhibitors transcripts in human and mouse. Talk Pacific Coast Protease Spring School, Borrego Springs, California, USA, April 2008.
  • Kappelhoff R., Puente, X.S., Seth, A., Lopez-Otin. C., and Overall, C.M. The Human Protease CLIP-CHIP: Genomic Analysis of all 721 Human Protease and Inhibitor Gene Transcripts in Human Tissue. Invited Talk. Degradomics. Systems Biology of the Protease Web Workshop, Edmonton, Alberta, May 8-9, 2006.
  • Kappelhoff, R. Puente, X.S., Seth, A., Lopez-Otin, C., and Overall, C.M. The CLIP-CHIP™: Genomic analysis and profiling of all 721 human protease and inhibitor gene transcripts. Talk Pacific Coast Protease Workshop, Desert Hot Springs, California, USA, April 2006.
  • Kappelhoff, R. Ewart, T., Puente, X.S., Seth, A., Lopez-Otin, C., and Overall, C.M. The human protease CLIP-CHIP: Genomic analysis of all 715 human protease and inhibitor gene transcripts in breast carcinomas. Talk Protease Workshop, Half Moon Bay, California, USA, April 2004. 

Poster Presentations

  • Kappelhoff, R., Dufour, A., Solis, N., and Overall, C.M. Proteomic identification of natural substrates for Matriptase-2″. 9th General Meeting of the International Proteolysis Society, Penang, Malaysia, October 4-8, 2015.
  • Kappelhoff, R., Dufour, A., Solis, N., Overall, C.M.Proteomic identification of natural substrates for Matriptase-2″. HUPO 14th Annual World Congress, Vancouver Canada, September 27-30, 2015.
  • Kappelhoff, R., Dufour, A., Kleifeld, O., Barré, O., auf dem Keller, U., and Overall, C.M. Proteomic identification of natural substrates for Matriptase-2.  7th Annual Earl W. Davie Symposium, Vancouver, BC, Canada, November 14, 2013.
  • Kappelhoff, R., Dufour, A., Kleifeld, O., Barré, O., auf dem Keller, U., and Overall, C.M. “Proteomic identification of natural substrates for Matriptase-2”.  8th General Meeting of the International Proteolysis Society, Cape Town, South Africa, October 20-24, 2013.
  • Kappelhoff, R., Puente, X.S., Wilson, C., Seth, A., Lopez-Otin, C., and Overall, C.M. “The CLIP-CHIP Microarray: Profiling all Proteases, Inhibitors and their homologues in man”. Canadian National Proteomics Network 5th Annual Symposium, Vancouver, BC, Canada, April 20-24, 2013.
  • Kappelhoff, R., Butler, G.S., Prudova, A., and Overall, C.M. What’s Up With Wild-type? Comparing Wild-type and Knock-out Mice on Transcriptomic and Proteomic level”. 7th General Meeting of the International Proteolysis Society, San Diego, California, USA, October 16-20, 2011.
  • Kappelhoff, R., Puente, X.S., Lopez-Otin, C., and Overall, C.M. Profiling USPs and other Intracellular Proteases with the Human and Murine CLIP-CHIP Microarray. Proteomics of Protein Degradation and Ubiquitin Pathways”. 1st PPDUP Conference, Vancouver, BC, Canada, June 6-8, 2010.
  • Kappelhoff, R., Puente, X.S., Wilson, C.H., Seth, A., Lopez-Otin, C., and Overall, C.M. “THE CLIP-CHIP Microarray: Tissue Profiling of all Proteases, Inhibitors and non-proteolytic Homologs in Man”. Gordon Research Conference. Proteolytic Enzymes and Their Inhibitors. Lucca (Barga), Italy. May 2 – 7, 2010.
  • Kappelhoff, R., Puente, X.S., Wilson, C.H., Seth, A., Lopez-Otin, C. “THE CLIP-CHIP Microarray: Tissue Profiling of all Proteases, Inhibitors and non-proteolytic Homologs in Man”. UBC Dentristry Research Day, Vancouver, BC, Canada, 26. January 2010.
  • Kappelhoff, R., Puente, X.S., Seth, A., Lopez-Otin, C., and Overall, C.M. “The CLIP-CHIP. Genomic Analysis and Profiling of all 721 Proteases, Inactive Homologues, and inhibitor Gene Transcripts in Human Tissues”. Poster Metzincin Metalloproteinases in Health and Disease Meeting, Centro Stefano Franscini, Monte Vérità, Ascona, Switzerland, Sept 24-29, 2006.
  • Kappelhoff R., Puente, X.S., Seth, A., Lopez-Otin. C., and Overall, C.M. “The human protease CLIP-CHIP: Genomic analysis of all 721 human protease and inhibitor gene transcripts in human tissue”. Poster 6th annual meeting of the Canadian Proteomic Initiative, Edmonton, Canada, May 2006.
  • Kappelhoff R., Puente, X.S., Seth, A., Lopez-Otin. C., and Overall, C.M. “The CLIP-CHIP: A dedicated and complete microarray for every human protease, inactive homologue and inhibitor”. Poster American Association for Cancer Research Special Conference on Cancer. Proteases and the Tumor Microenvironment, Bonita Springs, FL, USA, December 2005.
  • Kappelhoff R., Puente, X.S., Seth, A., Lopez-Otin. C., and Overall, C.M. “The CLIP-CHIP: A dedicated and complete microarray for every human protease, inactive homologue and inhibitor”. Poster 4th General Meeting of the International Proteolysis Society, Quebec City, Canada, October 2005.
  • Kappelhoff, R., Ewart, T., Puente, X.S., Seth, A., Lopez-Otin, C., and Overall, C.M. “The human protease CLIP-CHIP: Genomic analysis of all 715 human protease and inhibitor gene transcripts in human breast carcinoma”. Poster New Technologies, Novel Approaches in Proteomics Research. Michael Smith Laboratory, University of British Columbia, Vancouver, Canada, January 2005.
  • Kappelhoff R., Puente, X.S., Seth, A., Lopez-Otin. C., and Overall, C.M. “The CLIP Human Protease Chip: Genomic analysis of all 715 human protease and inhibitor gene transcripts in human breast carcinoma”. Poster CBR Reception, Centre for Blood Research Reception, University of British Columbia, Vancouver, Canada, September 2004.
  • Kappelhoff R., Puente, X.S., Seth, A., Lopez-Otin. C., and Overall, C.M. “The CLIP Human Protease Chip: Genomic analysis of all 715 human protease and inhibitor gene transcripts in human breast carcinoma”. Poster 95th annual meeting of the American Association for Cancer Research, Orlando, Florida, USA, March 2004.

 

Green Labs Progam

Green Labs promotional video (September 2014):

Original link: http://youtu.be/KLHH4y0YYJ4

Uploaded on Sep 16, 2014

The Green Labs Program offers sustainable solutions to reduce the environmental impact of research-related activities at UBC. Led collaboratively by Risk Management Services, Payment & Procurement Services, Campus + Community Planning and Health, Safety and Environment (UBC-Okanagan), the initiative offers researchers access to recycling and recovery programs, sustainability training and resources.

Music credit: The House Glows (With Almost No Help) By Chris Zabriskie